Holman Group Publications (1990-1999)

Clark A.E. and Holman, G.D. (1990) Exofacial photolabelling of the human erythrocyte glucose transporter with an azitrifluoroethyl-substituted bis-mannose. Biochem. J. 269, 615-622.


Calderhead, D.M., Kitagawa, K., Tanner, L.I., Holman, G.D. and Lienhard, G.E. (1990) Insulin Regulation of the two glucose transporters in 3T3-L1 adipocytes. J. Biol. Chem. 265, 13800-13808.


Holman, G.D., Kozka, I.J., Clark, A.E., Flower, C.J., Saltis, J., Habberfield, A.D., Simpson, I.A. and Cushman, S.W. (1990) Cell-surface labeling of glucose transporter isoform GLUT4 by bis-mannose photolabel: Correlation with stimulation of glucose transport in rat adipose cells by insulin and phorbol ester. J. Biol. Chem. 265, 18172-18179.


Gibbs, E.M., Calderhead, D.M., Holman, G.D. and Gould, G.W. (1991) Phorbol ester only partially mimics the effects of insulin on glucose transport and glucose-transporter distribution in 3T3-L1 adipocytes. Biochem. J. 275, 145-150.


Clark, A.E., Holman, G.D. and Kozka, I.J. (1991) Determination of the rates of appearance and loss of glucose transporters at the cell surface of rat adipose cells. Biochem. J. 278, 235-241.


Kozka, I.J., Clark, A.E. and Holman, G.D. (1991) Chronic treatment with insulin selectively down-regulates cell-surface GLUT4 in 3T3-L1 adipocytes. J. Biol. Chem. 266, 11726-11731.


Yang, J., Clark, A.E., Harrison, R., Kozka, I.A. and Holman, G.D. (1992) Trafficking of glucose transporters in 3T3-L1 cells: Inhibition of trafficking by phenylarsine oxide implicates a slow dissociation of transporters from trafficking proteins. Biochem J. 281, 809-817


Palfreyman, R.W., Clark, A.E., Denton, R.M., Holman, G.D. and Kozka, I.J. (1992) Kinetic resolution of the separate GLUT1 and GLUT4 glucose transport activities in 3T3-L1 cells. Biochem. J. 284, 275-281


Yang, J., Clark, A.E., Kozka, I.J., Cushman, S.W. and Holman, G.D. (1992) Development of an intracellular pool of glucose transporters in 3T3-L1 cells. J. Biol. Chem. 267, 10393-10399


Jordan, N. and Holman, G.D. (1992) Labelling of the liver-type glucose transporter by an azitrifluroethyl-benzoyl substituted bis-mannose. Biochem. J. 286, 649-656


Hashiramoto, M., Kadowaki, T., Clark, A.E., Muraoka, A., Momomura, K., Sakura, H., Tobe, K., Akanuma, Y., Yazaki, Y., Holman, G.D. and Kasuga, M. (1992) Site-directed mutagenesis of GLUT1 in helix 7 results in perturbation of exofacial ligand binding. J. Biol. Chem. 267, 17502-17507


Vannucci, S.J., Nishimura, H., Satoh, S., Cushman, S.W.,  Holman, G.D. and Simpson, I.A. (1992) Changes in cell-surface accessibility of GLUT4 glucose transporter: Effect of insulin, isoproterenol and adenosine  Biochem. J. 288, 325-330


Stagsted, J., Ziebe, S., Satoh, S., Holman, G.D., Cushman, S.W. and Olsson, J. (1993) Insulinomimetic effect of epidermal growth factor when combined with an MHC-Class1-derived peptide. J Biol. Chem. 268, 1770-1774


Yang, J and Holman, G.D. (1993) Comparison of GLUT4 and GLUT1 subcellular trafficking in basal and insulin-stimulated 3T3-L1 cells. J. Biol. Chem. 268, 4600-4603


Satoh, S., Nishimura, H., Clark, A.E., Kozka, I.J., Vannucci, S.J., Simpson, I.A., Cushman, S.W. and Holman, G.D. (1993) Use of bis-mannose photolabel to elucidate insulin-regulated GLUT4 subcellular trafficking kinetics in rat adipose cells : Evidence that exocytosis is critical site of hormone action. J. Biol. Chem. 269, 17820-17829


Kozka, I.J. and Holman, G.D. (1993) Metformin blocks down-regulation of cell-surface GLUT4 caused by chronic-insulin treatment of rat adipocytes. Diabetes 42, 1159-1165


Fry, A.J., Towner, P., Holman, G.D. and Eisenthal, R. (1993) Transport of D-fructose and its analogues by Trypanosoma brucei. Mol. Biochem. Parasitol. 60, 9-18


Gould, G.W. and Holman, G.D. The glucose transporter family: structure, function and tissue-specific expression (1993) Biochem. J. 295, 329-341


Stagsted, J., Olsson, L., Holman, G.D., Cushman, S.W. and Satoh, S. (1993) Inhibition of internalization of glucose transporters and IGF-II receptors: Mechanism of action of MHC Class 1-derived peptides which augment the insulin response in rat adipose cells. J. Biol. Chem. 268, 22809-22813


Lund, S., Holman, G.D., Schmitz, O. and Pedersen, O. (1993) GLUT4 content in the plasma membrane of rat skeletal muscle: Comparative studies of the subcellular fractionation method and exofacial the exofacial photolabeling technique using ATB-BMPA. FEBS Lett. 330, 312-318


Dudek, R.W., Wilson, C., Holman, G.D., Cushman, S.W. and Dohm, G.L. (1994) Glucose transporter localization in rat skeletal muscle: autoradiographic study using ATB-[2-3H]-BMPA photolabel. FEBS Lett. 339, 205-208


Cope, D.L., Holman, G.D., Baldwin, S.A. and Wolstenholme, A.J. (1994) Domain assembly of the GLUT1 glucose transporter. Biochem. J. 300, 291-294


Tamori, Y., Hashiramoto, M., Clark, A.E., Mori, H., Muraoka, A., Kadowaki, T., Holman, G.D. and Kasuga, M. (1994) Substitution at Pro385 of GLUT1 perturbs the glucose transport function by reducing conformational flexibility. J. Biol. Chem. 269, 2982-2986


Mori, H., Hashiramoto, M., Clark, A.E., Yang, J., Muraoka, A., Tamori, Y., Kasuga, M. and Holman, G.D. (1994) Substitution of Tyr293 of GLUT1 locks the transporter into an outward-facing conformation.  J. Biol. Chem. 269, 11578-11583


Holman, G.D., Lo Leggio, L., Cushman, S.W. (1994) Multiple pool membrane protein trafficking: Application to insulin stimulated glucose transporter recycling. J. Biol. Chem. 269, 17516-17524


Clark, J.F., Young, P.W., Yonezawa, K., Kasuga, M. and  Holman, G.D. (1994) Inhibition of the translocation of GLUT1 and GLUT4 in 3T3-L1 cells by the PI 3-kinase inhibitor, Wortmannin. Biochem. J. 300, 631-635


Gould, G.W., Merral, N.W., Martin, S., Jess, T.J., Campbell, I.W., Calderhead, D.M., Gibbs, E.M., Holman, G.D. and Pleven, R.J. (1994) Growth factor induced stimulation of hexose transport in 3T3-L1 adipocytes. Evidence that insulin-induced translocation of GLUT4 is independent of activation of MAP kinase. Cell. Signal. 6, 313-320


Hara, K., Yonezawa, K., Sakaue, H., Ando, A., Kotani, K., Kitamura, T., Ido, Y., Ueda, H., Stephens, L., Jackson, T., Hawkins, P.T., Dhand, R., Clark, A.E., Holman, G.D., Waterfield, M.D., and Kasuga, M. (1994) Phosphatidylinositol 3-kinase activity is required for insulin-stimulated glucose transport but not Ras activation in CHO cells. Proc. Natl. Acad. Sci. U.S.A. 91, 7415-7419


Holman, G.D. and Cushman, S.W. (1994) Subcellular localization and trafficking of the GLUT4 glucose transporter isoform in insulin-responsive cells Bioessays 16, 753-759


Lund, S., Flyvbjerg, A., Holman, G.D., Pedersen, O., and Schmitz, O. (1994) Comparative effects of IGF1 and insulin on the glucose transporter system in isolated rat soleus muscle. Am. J. Physiol. 267, 461-466


Kozka, I.J., Clark, A.E., Reckless, J.P.D., Cushman, S.W., Gould, G.W., and Holman, G.D. (1995) The effects of insulin on the level and activity of the GLUT4 present in human adipose cells. Diabetologia 38, 661-666


Lund, S., Holman, G.D., Schmitz, O and Pedersen, O. (1995) Contraction stimulates translocation of glucose transporter GLUT4 in skeletal muscle through a mechanism distinct from that of insulin. Proc. Natl. Acad. Sci. U.S.A. 92, 5817-5821


Mueckler, M. and Holman, G. (1995) Glucose transport- Homeostasis without a GLUT Nature 377, 100-101


Muraoka, A., Hashiramoto, M., Clark, A.E., Sakura, H., Kadowaki, T., Holman, G.D. and Kasuga, M. (1995) Analysis of the structural features of the C-terminus of GLUT1 that are required for transport catalytic activity. Biochem J. 311, 699-704


Young, P.W., Cawthorne, M.A., Coyle, P.J., Holder, J.C., Holman, G.D., Kozka, I.J., Kirkham D., and Smith, S.A. (1995) Repeat treatment of obese mice with BRL 49653, a new and potent insulin sensitizer, enhances insulin action in white adipose cells- association with increased insulin binding and cell surface GLUT4 as measured by photoaffinity labelling Diabetes 44, 1087-1092


Yang, J., Clarke, J.F., Ester, C.J., Young, P.W., Kasuga, M. and Holman, G.D. (1996) Phosphatidylinositol-3-kinase acts at an intracellular membrane site to enhance GLUT4 exocytosis in 3T3-L1 cells. Biochem. J. 313, 125-131


Araki, S., Yang, J., Hashiramoto, M., Tamori, Y., Kasuga, M. and Holman, G.D. (1996) Subcellular trafficking kinetics of GLUT4 mutated at the N- and C-termini. Biochem. J. 315, 153-159


Fischer, Y., Thomas, J., Holman, G.D., Rose, H., and Kammermeier, H. (1996) Contraction independent effects of catecolamines on glucose transport in isolated rat cardiomyocytes. Am. J. Physiol. 39, C1204-C1210


Holman, G.D. and Cushman, S.W. (1996) Subcellular trafficking of GLUT4 in insulin target cells. Seminars in Cell and Developmental  Biol. 7, 259-268


Cusdin, F.S., Robinson, M.J., Holman, G.D., Hough, D.W., Danson, M.J. (1996) Characterisation of glucose transport in the hyerthermophilic archaeon Sulfolobus solfatarius FEBS Lett. 315, 153-159


Timmers, K.I., Clarke, A.E., Omatsu-Kanbe, M., Whiteheart, S.W., Bennett, M.K., Holman, G.D., and Cushman, S.W. (1996). Identification of SNAP receptors in rat adipose cell membrane fractions and in SNARE complexes co-immunoprecipitated with epitope-tagged N-ethyl-maleimide senstive fusion protein. Biochem. J. 320, 429-436


Fischer, Y., Thomas, J., Sevilla, L., Munoz, P., Becker, C., Holman, G.D., Kozka, I.J., Palacin, M., Testar, X., Kammermeier, H. and Zorzano, A. (1997) Insulin induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes - evidence of the existence of different intracellular GLUT4 vesicle populations. J. Biol. Chem. 272, 7085-7092


Holman, G.D. and Kasuga, M. (1997) From Receptor to Transporter: Insulin Signalling to Glucose Transport. Diabetologia. 40, 991-1003


Lund, S., Holman, G.D., Zierath, J.R., Rincon, J., Nolte, L.A., Clark, A.E., Schmitz, O., Pedersen, O., and Wallberg-Henriksson, H. (1997) Effect of insulin on GLUT4 translocation and turnover rate in human skeletal muscle as measured by the exofacial bismannose photolabeling technique. Diabetes 46, 1965-1969


Koumanov, F.,  Yang, J.,  Jones, A.E., Hatanaka Y., and Holman, G.D (1998) Cell surface biotinylation of GLUT4 using bis-mannose photolabels. Biochem. J. 330, 1205-1215


Doege, H., Schurmann, A., Ohnimus, H., Monser, V., Holman, G.D. and Joost, H.G. (1998) Serine-294 and threonine-295 in the exofacial loop domain between helices 7 and 8 of glucose transporters are involved in the conformational alterations during the transport process. Biochem.J. 329, 289-293


Lund, S., Pryor, P.R., Ostergaard, S., Schmitz, O., Pedersen, O. and Holman, G.D.. (1998) Evidence against protein kinase B as a mediator of contraction- induced glucose transport and glut4 translocation in rat skeletal muscle. FEBS Lett. 425, 472-474

Etgen, J.G., Zavadoski, W.J., Holman, G.D. and Gibbs, E.M. (1999) Insulin-sensitive regulation of glucose transport and GLUT4 translocation in skeletal muscle of GLUT1 transgenic mice.  Biochem.J. 337, 51-57.

Simpson, I.A., Appel, N.M. , Hokari, M., Oki, J.,  Holman, G. D., Maher, F., KoehlerStec, E.M., Vannucci, S.J. and Smith Q. R. (1999) Blood-brain barrier glucose transporters: Effects of hypo- and hyperglycemia. J.Neurochem. 72, 238-247

Abbadi, M., Holman, G.D., Morin, C., Rees, W.D. and Yang, J.. (1999) Synthesis of symmetrical 4,4 '- and 6,6 '- bis(D-glucose)-based probes as tools for the study of D-glucose transport proteins. Tetrahedron Lett. 40, 5861-5864

Ryder J.W., Kawano Y., Chibalin A.V., Rincon J, Tsao T.S., Stenbit A.E., Combatsiaris T., Yang J., Holman G.D., Charron M.J. and Zierath J.R. (1999) In vitro analysis of the glucose-transport system in GLUT4-null skeletal muscle. Biochem. J. 342, 321-328

Derave W., Lund S., Holman G.D., Wojtaszewski J., Pedersen O. and Richter E.A. (1999) Contraction-stimulated muscle glucose transport and GLUT-4 surface content are dependent on glycogen content. Am. J. Physiol. 277, E1103-E1110

Holman G.D. (1999) Regulated exocytosis: A new deadly Syn? Current Biol. 9, R735-R737

Gillingham A.K., Koumanov F., Pryor P. R., Reaves B.J. and Holman G.D. (1999) Association of AP1 adaptor complexes with GLUT4 vesicles. J. Cell Sci. 112, 4793-4800